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Dear Colleague,

Continuing my dialogue from the previous newsletter, one of the pitfalls of convergence is right implementation and governance. While governance looks at the overall benefit, some specific benefits are lost and hence may create a setback. The analogy I draw is that ‘if we want high specificity, then a compromise on sensitivity is a given.’ However, the difference we can make is by knowing what we don’t know and what we know. To share this knowledge among our peers, we have launched a column named “what not”. This column is intended to identify pitfalls and bring them into the open; there may or may not be a solution today, but I believe that thinking about the problem is a start to solving the problem. I am sure, with your experience in this industry, you have many such pointers and I would be happy to receive your “what not”s and include them in the forthcoming editions. I am glad that our customers and partners have started contributing to this communiqué and this makes it merrier. What better compliment than the endorsement?

Best regards,
Naveen Kulkarni

Hotspots to Activity - Get to know your enzyme mechanism and "otherwise" unknown facts by applying eEF
- Pravin Kumar
Enzymes are exquisitely evolving catalytic machineries that can speed up a chemical reaction up to 17 logs (Table 1). In retrospect, the enzyme tends to confront when taken-off from its home ground and placed in a fermentor. However, engineering enzymes by incorporating amino acid substitutions both rationally and irrationally has enabled the evolution of a new class of enzymes that Read more…
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Partner speak...

Importance of stem cells: A surgeon’s viewpoint   ePrime: A powerful design engine with an intuitive interface
- Dr. Prabhakar
Ophthalmologist, JSS University, Mysore.
  - Dr. Dale Kunde
Lecturer, University of Tasmania, Australia
Progressive pterygium is observed frequently in young and middle aged individuals who have more potential for recurrence after the excision than atrophic pterygium. It is clinically constituted by the proliferation of fibrovascular components apparently emanating from the medial canthus invading towards the cornea. The disease is multifactorial, mostly Read more…

  The explosion of molecular techniques utilising PCR over the recent decades has driven the need for more accurate algorithms and software for design of oligonucleotide primers. Good primer design is imperative for simple PCR, real-time PCR and the newer technologies such as Dual-labelled hydrolysis (Taqman) and Molecular Beacon assays which involve the concomitant Read more…
In the news...
TATAA Biocenter announces to use & resell the new version of ePrime™
TATAA Biocenter, the world’s largest provider of hands-on training in qPCR and Sweden’s most comprehensive distributor of qPCR products, today announced a reseller agreement with Polyclone Bioservices to distribute ePrime™ – a qPCR primer & probe design application and also, to introduce ePrime™ in its qPCR courses. Developed  primer design experience by adopting recent technology advancements in its new version (4.0), to highlight meaningful & valuable aspects of primer design. ePrime™ offers a host of unparallel design features including the ability to save design criteria for future work, customizable design reports in MS Excel® format and a smart Read more…
Polyclone signs MoU with JSS University
JSS MoU with Polyclone
Polyclone Bioservices Pvt. Ltd. (Polyclone) signed a memorandum of understanding (MoU) with JSS University, Mysore on 4th September 2012. This MoU establishes the
framework for collaborative research and development, in biological sciences between two organizations. JSS University, Mysore, is an institute of higher learning, offering educational programs in the area of Arts, Science, and Commerce & Business Management. JSS, Mysore is widely recognized for the quality of its undergraduate, graduate and professional educational programs. Collaborations under this MOU are focused on collaborative Read more…
What Not
- Vasanthi & Pravin

• Do not store cord blood at room temperature if it has to be processed 24-48 hours after collection. If cord blood is to be stored before processing and cryopreservation, it is preferable to store at 4°C which will significantly improve the cell recovery and viability when compared to units stored at room temperature (Louis I, Impact of storage temperature and processing delays on cord blood quality: Transfusion. 2012 ).

• Do not use the protein structures as such from PDB for molecular modelling studies, since majority of the PDB structures have problems such as missing hydrogen atoms, inadequate information on connectivity, bond orders & formal charges of some atoms, incomplete side chains & loops etc. Hence, using tools like Modeller, model the most appropriate positions of hydroxyl and thiol hydrogens, protonation states & tautomers of histidine residues, missing side chains & loops etc.